Anticorps Recombinant de lapin anti-Phospho-SH3PXD2B (Ser291)

Phospho-SH3PXD2B (Ser291) Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

241064C5

N° de cat : 84563-1-RR

Synonymes

Phospho-SH3PXD2B, Phospho-SH3PXD2B Ser291, P-SH3PXD2B, P-SH3PXD2B (Ser291), SH3PXD2B (Ser291)

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Galerie de données de validation

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  WB analysis using 84563-1-RR

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 84563-1-RR (Phospho-SH3PXD2B (Ser291) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (66305-1-Ig) antibody as a loading control.

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  IF Staining of HeLa using 84563-1-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed λ phosphatase treated HeLa cells using Phospho-SH3PXD2B (Ser291) antibody (84563-1-RR, Clone: 241064C5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HeLa traitées à la λ phosphatase
• Résultats positifs en IF/ICC: cellules HeLa traitées à la λ phosphatase,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84563-1-RR cible Phospho-SH3PXD2B (Ser291) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SH3 and PX domains 2B
• Masse moléculaire calculée: 911aa, 102 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_001017995
• Symbole du gène: SH3PXD2B
• Identification du gène (NCBI): 285590
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SH3 and PX domains 2B (SH3PXD2B) is also known as tyrosine kinase substrate with four SH3 domains (Tks4). It consists of the N-terminal phox homology domain, four Sr homology 3 domains, several proline-rich regions, and Src phosphorylation sites. Previous studies have shown that SH3PXD2B is an essential invadopodium scaffold protein and can stimulate actin polymerization, recruit membrane type-1 matrix metalloproteinase (MT1-MMP) to intracellular membranes, and increase Nox1-dependent reactive oxygen species (ROS) production. (PMID: 33906640)

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