Anticorps Recombinant de lapin anti-Phospho-SMAD1 (Ser195)

Phospho-SMAD1 (Ser195) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1K6

N° de cat : 80257-1-PBS

Synonymes

p SMAD1, p SMAD1 (Ser195), p SMAD1 Ser195, Phospho SMAD1, Phospho SMAD1 (Ser195)

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Galerie de données de validation

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  WB analysis using 80257-1-RR (same clone as 80257-1-PBS)

  Non-treated and UV treated A431 cells were subjected to SDS PAGE followed by western blot with 80257-1-RR (Phospho-SMAD1 (Ser195) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80257-1-PBS in a different storage buffer formulation.

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  WB analysis using 80257-1-RR (same clone as 80257-1-PBS)

  Non-treated and BMP2 (HZ-1128) treated HepG2 cells were subjected to SDS PAGE followed by western blot with 80257-1-RR (Phospho-SMAD1 (Ser195) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80257-1-PBS in a different storage buffer formulation.

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Informations sur le produit

80257-1-PBS cible Phospho-SMAD1 (Ser195) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 1
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC001878
• Symbole du gène: SMAD1
• Identification du gène (NCBI): 4086
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

SMAD family member 1 (Smad1) have been involved in metastatic progression of many cancer types. Smad1 can be induced by many tumor-stimulating cytokines such as the bone morphogenetic protein 2 (BMP2) and TNFα and plays important roles in cell invasion and metastasis. BMP2 signalling is initiated by binding to its specific receptors, which leads to in the phosphorylation and nuclear translocation of Smad1. Translocated Smad1 then modulates the expression of downstream osteogenic genes. (PMID: 32954678, PMID: 30008908)

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