Anticorps Recombinant de lapin anti-Phospho-SMAD9 (Ser465/467)

Phospho-SMAD9 (Ser465/467) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240470A12

N° de cat : 80144-2-RR

Synonymes

P SMAD5 (Ser463/465), p SMAD9, Phos SMAD1 (Ser463/465), Phos SMAD9, Phospho SMAD9 (Ser465/467)

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Galerie de données de validation

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  WB analysis using 80144-2-RR

  Non-treated and BMP2 (HZ-1128) treated HeLa cells were subjected to SDS PAGE followed by western blot with 80144-2-RR (Phospho-SMAD9 (Ser465/467) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: BMP2 treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80144-2-RR cible Phospho-SMAD9 (Ser465/467) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: SMAD family member 9
• Masse moléculaire calculée: 430 aa, 49 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC011559
• Symbole du gène: SMAD9
• Identification du gène (NCBI): 4093
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mothers against decapentaplegic homolog 9 also known as SMAD9, SMAD8, and MADH6, belongs to the SMAD family of proteins, which belong to the TGFβ superfamily of modulators. Like many other TGFβ family members, SMAD9 is involved in cell signalling. When a bone morphogenetic protein binds to a receptor (BMP type 1 receptor kinase) it causes SMAD9 to interact with SMAD anchor for receptor activation (SARA).The binding of ligands causes the phosphorylation of the SMAD9 protein and the dissociation from SARA and the association with SMAD4. It is subsequently transferred to the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD9 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase. There are two isoforms of the protein. The antibody recognizes both SMAD1 and SMAD5 protein phosphorylated at Ser463/465.

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