Anticorps Polyclonal de lapin anti-Phospho-STAT1 (Ser727)

Phospho-STAT1 (Ser727) Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

2

Avis (1)

N° de cat : 28977-1-AP

Synonymes

DKFZp686B04100, ISGF 3, Phospho-STAT1 (Ser727）, STAT1, STAT91

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Galerie de données de validation

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  WB analysis using 28977-1-AP

  Non-treated HeLa cells and IFN gamma treated HeLa cells were subjected to SDS PAGE followed by western blot with 28977-1-AP (Phospho-STAT1 (Ser727) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of HeLa using 28977-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed IFN alpha treated HeLa cells using Phospho-STAT1 (Ser727) antibody (28977-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à l'IFN gamma,
• Résultats positifs en IF: IFN alpha treated HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

28977-1-AP cible Phospho-STAT1 (Ser727) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 1, 91kDa
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 84 kDa
• Numéro d’acquisition GenBank: BC002704
• Symbole du gène: STAT1
• Identification du gène (NCBI): 6772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT1 activating phosphorylation at Ser727 is p38-dependant during lipotoxic stress. MLK3 activation triggers a MAPK signaling cascade resulting in STAT1 Ser727 phosphorylation. The phosphorylation of STAT1 Ser727 increases its nuclear localization, and transcriptional activity. (PMID: 28262979).

Publications

Species	Application	Title
human	WB	Res Sq; The RhoA GTPase regulates Type I Interferon Signaling in Systemic lupus erythematosus; Authors - Wei Fan
human	WB	Cancer Res Commun; Differential Regulation of the Stimulator of Interferon Genes pathway in Human Papillomavirus positive and negative Head and Neck Cancers; Authors - Emma Saulters

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (11-24-2022)

Works well for western blots. Only had a few unspecific bands but the antibody showed nice results for a phosphorylated protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Human cell lines