Anticorps Polyclonal de lapin anti-Phospho-STAT1 (Tyr701)

Phospho-STAT1 (Tyr701) Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 28979-1-AP

Synonymes

p STAT1, p STAT1 (Tyr701), p STAT1 Tyr701, Phospho STAT1, Phospho STAT1 (Tyr701)

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Galerie de données de validation

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  WB analysis using 28979-1-AP

  Non-treated THP-1 cells, IFN gamma and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28979-1-AP (Phospho-STAT1 (Tyr701) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 28979-1-AP

  Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using Phospho-STAT1 (Tyr701) antibody (28979-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  FC experiment of THP-1 using 28979-1-AP

  1x10^6 THP-1 cells untreated (dashed line) or treated with IFN gamma (red) were intracellularly stained with 0.25 ug Phospho-STAT1 (Tyr701) Polyclonal antibody (28979-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: IFN gamma and LPS treated THP-1 cells
• Résultats positifs en IF/ICC: IFN alpha treated HeLa cells,
• Résultats positifs en FC (Intra): IFN gamma treated THP-1 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

28979-1-AP cible Phospho-STAT1 (Tyr701) dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 1, 91kDa
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 84-91 kDa
• Numéro d’acquisition GenBank: BC002704
• Symbole du gène: STAT1
• Identification du gène (NCBI): 6772
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).

Publications

Species	Application	Title
human	WB	J Transl Med; Pretreatment of UC-MSCs with IFN-α2 improves treatment of liver fibrosis by recruiting neutrophils; Authors - Ye Xie
human	WB	mBio; AUP1 and UBE2G2 complex targets STING signaling and regulates virus-induced innate immunity; Authors - Xin-Tao Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Agnieszka (Verified Customer) (02-28-2023)

great working antibody, gives the background

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000

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