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Anticorps Recombinant de lapin anti-Phospho-STAT1 (Tyr701)

Phospho-STAT1 (Tyr701) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1C5

N° de cat : CL488-82674-10

Synonymes

p STAT1, p STAT1 (Tyr701), p STAT1 Tyr701, Phospho STAT1, Phospho STAT1 (Tyr701)

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of THP-1 cells using CoraLite® Plus 488-conjugated Phospho-STAT1 (Tyr70 (CL488-82674-10)

    FC experiment of THP-1 using CL488-82674-10

    1X10^6 THP-1 cells untreated (dashed lines) or treated with IFN gamma which intracellularly stained with 0.25 ug Coralite® Plus 488 Phospho-Stat1 (Tyr701) Recombinant Antibody (CL488-82674-10, Clone:1C5)(red), or 0.25 ug CoraLite® Plus 488 Rabbit IgG Isotype Control RecAb (CL488-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)IFN gamma treated THP-1 cells,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-82674-10 cible Phospho-STAT1 (Tyr701) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet signal transducer and activator of transcription 1, 91kDa
Masse moléculaire calculée 83 kDa
Poids moléculaire observé84-91 kDa
Numéro d’acquisition GenBankBC002704
Symbole du gène STAT1
Identification du gène (NCBI) 6772
Conjugaison CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. STAT family members are phosphorylated by the receptor associated kinases, and then form homo-or heterodimers that translocate to the cell nucleus where they act as transcription activators. The phosphorylation of STAT1 at Tyr701 promotes the dimerization of STAT1 and its translocation into the nucleus where it binds the GAS promoter to allow the transcription of inflammatory response genes(PMID: 31408005). The transcript of STAT1 undergoes alternative splicing, resulting in two isoforms: STAT1α (91 kDa) and STAT1β (84 kDa)(PMID: 20159032).

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