Anticorps Polyclonal de lapin anti-Phospho-STAT3 (Ser727)

Phospho-STAT3 (Ser727) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(22)

N° de cat : 28945-1-AP

Synonymes

p STAT3, p STAT3 (Ser727), p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727)

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Galerie de données de validation

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  WB analysis using 28945-1-AP

  Phosphatase inhibitor treated HEK-293T cells, λ phosphatase treated HEK-293T cells, and non-treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28945-1-AP (Phospho-STAT3 (Ser727) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293T traitées à la λ phosphatase

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 22 publications below
• IF: See 2 publications below

Informations sur le produit

28945-1-AP cible Phospho-STAT3 (Ser727) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, bovin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 3 (acute-phase response factor)
• Masse moléculaire calculée: 770 aa, 88 kDa
• Poids moléculaire observé: 92-100 kDa
• Numéro d’acquisition GenBank: BC000627
• Symbole du gène: STAT3
• Identification du gène (NCBI): 6774
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. Phosphorylated STAT3 dimerizes and translocates to the nucleus, where it regulates the transcription of target genes through binding to specific DNA-responsive elements. In addition to activation by Tyr705 phosphorylation, STAT3 also requires phosphorylation on Ser727 to achieve maximal transcriptional activity. (PMID: 21617181)

Publications

Species	Application	Title
mouse	WB	Int Immunopharmacol; The malic acid inhibiting inflammation in ankylosing spondylitis by interfering M1 macrophage polarization; Authors - Zhou Ji
mouse	WB	Bioorg Chem; N-Heterocyclic functionalized chalcone derivatives as anti-inflammatory agents for atopic dermatitis treatment by inhibiting JAK1/STAT3 signaling pathway; Authors - Zhengxiao Huang
human	WB	World J Surg Oncol; Targeting NETO2 suppresses cell proliferation, invasion, and migration and inactivates the STAT3/C-MYC pathway in hepatocellular carcinoma; Authors - Na Shun Meng He
human	WB	Biochem Pharmacol; N6-methyladenosine-modified RNF220 induces cisplatin resistance and immune escape via regulating PDE10A K48-linked ubiquitination in bladder cancer; Authors - Kai Li
mouse	WB	Genes Dis; Inhibition of sphingosine 1-phosphate receptor 3 ameliorates bleomycin-induced pulmonary fibrosis by suppressing macrophage M2 polarization; Authors - Huijun Qiu
mouse	WB	Cancer Immunol Immunother; Dual efficacy of tocilizumab in managing PD-1 inhibitors-induced myocardial inflammatory injury and suppressing tumor growth with PD-1 inhibitors: a preclinical study; Authors - Yanxin Chen

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