Anticorps Polyclonal de lapin anti-Phospho-STAT3 (Ser727)
Phospho-STAT3 (Ser727) Polyclonal Antibody for WB, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (3)
Applications
WB, IF, ELISA
Conjugaison
Non conjugué
N° de cat : 28945-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules HEK-293T, cellules HEK-293T traitées à la λ phosphatase |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 22 publications below |
| IF | See 2 publications below |
Informations sur le produit
28945-1-AP cible Phospho-STAT3 (Ser727) dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | rat, bovin, Humain, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | signal transducer and activator of transcription 3 (acute-phase response factor) |
| Masse moléculaire calculée | 770 aa, 88 kDa |
| Poids moléculaire observé | 92-100 kDa |
| Numéro d’acquisition GenBank | BC000627 |
| Symbole du gène | STAT3 |
| Identification du gène (NCBI) | 6774 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. Phosphorylated STAT3 dimerizes and translocates to the nucleus, where it regulates the transcription of target genes through binding to specific DNA-responsive elements. In addition to activation by Tyr705 phosphorylation, STAT3 also requires phosphorylation on Ser727 to achieve maximal transcriptional activity. (PMID: 21617181)
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-STAT3 (Ser727) antibody 28945-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int Immunopharmacol The malic acid inhibiting inflammation in ankylosing spondylitis by interfering M1 macrophage polarization | ||
Bioorg Chem N-Heterocyclic functionalized chalcone derivatives as anti-inflammatory agents for atopic dermatitis treatment by inhibiting JAK1/STAT3 signaling pathway | ||
World J Surg Oncol Targeting NETO2 suppresses cell proliferation, invasion, and migration and inactivates the STAT3/C-MYC pathway in hepatocellular carcinoma | ||
Biochem Pharmacol N6-methyladenosine-modified RNF220 induces cisplatin resistance and immune escape via regulating PDE10A K48-linked ubiquitination in bladder cancer | ||
Genes Dis Inhibition of sphingosine 1-phosphate receptor 3 ameliorates bleomycin-induced pulmonary fibrosis by suppressing macrophage M2 polarization | ||
Cancer Immunol Immunother Dual efficacy of tocilizumab in managing PD-1 inhibitors-induced myocardial inflammatory injury and suppressing tumor growth with PD-1 inhibitors: a preclinical study |
