Anticorps Monoclonal anti-Phospho-STAT3 (Ser727)

Phospho-STAT3 (Ser727) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2G11G11

N° de cat : 60479-1-Ig

Synonymes

p STAT3 (Ser727), p STAT3, p STAT3 Ser727, Phospho STAT3, Phospho STAT3 (Ser727)

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Galerie de données de validation

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  WB analysis using 60479-1-Ig

  UV treated and untreated HeLa cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

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  WB analysis using 60479-1-Ig

  EGF treated and untreated A549 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

• 

  WB analysis using 60479-1-Ig

  TNF alpha (HZ-1014) treated and untreated MCF-7 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

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  WB analysis using 60479-1-Ig

  TNF alpha (HZ-1014) treated and untreated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (Phospho-STAT3 (Ser727) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with HRP-conjugated Alpha Tubulin (HRP-66031) antibody as a loading control.

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  WB analysis of HSC-T6 using 60479-1-Ig

  UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 60479-1-Ig (STAT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 60479-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Phospho-STAT3 (Ser727) antibody (60479-1-Ig, Clone: 2G11G11 ) at dilution of 1:500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  IF Staining of HeLa using 60479-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed untreated HeLa cells, UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells using Phospho-STAT3 (Ser727) antibody (60479-1-Ig, Clone: 2G11G11) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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  FC experiment of HeLa using 60479-1-Ig

  1x10^6 untreated HeLa cells (dash lines), UV (1 hour) and 100 nM Calyculin A (30 minutes) treated HeLa cells (full lines) were intracellularly stained with 0.1 μg Phospho-STAT3 (Ser727) Monoclonal antibody (60479-1-Ig, Clone: 2G11G11, red) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM005). Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2, blue) was parallel stained as control. Cells were fixed with 4% PFA .

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules MCF-7, cellules NIH/3T3
• Résultats positifs en IF/ICC: UV(1hour),100 nM Calyculin A(30 minutes)treated HeLa cells, cellules HeLa
• Résultats positifs en FC (Intra): UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.10 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

60479-1-Ig cible Phospho-STAT3 (Ser727) dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 3 (acute-phase response factor)
• Masse moléculaire calculée: 770 aa, 88 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: BC000627
• Symbole du gène: STAT3
• Identification du gène (NCBI): 6774
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3, synonyms: APRF, FLJ20882, MGC16063) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT3 is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.

Publications

Species	Application	Title
human	WB	Redox Biol; iPSC-derived cardiomyocytes and engineered heart tissues reveal suppressed JAK2/STAT3 signaling in LMNA-related emery-dreifuss muscular dystrophy; Authors - Hangping Fan
rat	WB	Stem Cell Res Ther; Bone marrow mesenchymal stem cells alleviate liver fibrosis after rat liver transplantation through JAK1/STAT5 pathway; Authors - Zhuyuan Si

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