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Anticorps Recombinant de lapin anti-Phospho-STAT5A (Ser726)/STAT5B (Ser731)

Phospho-STAT5A (Ser726)/STAT5B (Ser731) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240709D6

N° de cat : CL647-80138-2

Synonymes

p STAT5 (Ser726), p STAT5A, p STAT5A (Ser726), p STAT5A Ser726, Phospho STAT5 (Ser726)

Galerie de données de validation

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  • Flow cytometry (FC) experiment of TF-1 cells using CoraLite® Plus 647-conjugated Phospho-STAT5A (Ser7 (CL647-80138-2)

    FC experiment of TF-1 using CL647-80138-2

    1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Stat5a (Ser726)/Stat5b (Ser731) Recombinant Antibody (CL647-80138-2, Clone:240709D6)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules TF-1 traitées au GM-CSF,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-80138-2 cible Phospho-STAT5A (Ser726)/STAT5B (Ser731) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet signal transducer and activator of transcription 5A
Masse moléculaire calculée 794 aa, 92 kDa
Poids moléculaire observé90-95 kDa
Numéro d’acquisition GenBankBC027036
Symbole du gène STAT5A
Identification du gène (NCBI) 6776
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5 proteins consisting of STAT5A and STAT5B are the main targets of IL-3 signaling. Upon cytokine stimulation, JAK2 phosphorylates STAT5 and the phosphorylated STAT5 proteins dimerize and translocate into the nucleus, where, by binding DNA, they activate target genes including c-fos. In addition to this JAK-catalyzed tyrosine phosphorylation, STAT5 may undergo serine phosphorylation in the carboxy-terminal P(M)SP site in response to prolactin. (PMID: 15795318)

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