Anticorps Recombinant de lapin anti-Phospho-STAT5A (Tyr694)/STAT5B (Tyr699)

Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(8)

CloneNo.

2P21

N° de cat : 80115-1-RR

Synonymes

Phospho-STAT5A (Tyr694), STAT5A, STAT5, MGF, 2P21

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Galerie de données de validation

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  WB analysis using 80115-1-RR

  Non-treated TF-1 and GM-CSF (HZ-1002) treated TF-1 cells were subjected to SDS PAGE followed by western blot with 80115-1-RR (Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control.

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  FC experiment of TF-1 using 80115-1-RR

  1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF which intracellularly stained with 0.06 ug Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) Recombinant antibody (80115-1-RR, Clone:2P21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules TF-1 traitées au GM-CSF,
• Résultats positifs en FC (Intra): cellules TF-1 traitées au GM-CSF,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IHC: See 1 publications below

Informations sur le produit

80115-1-RR cible Phospho-STAT5A (Tyr694)/STAT5B (Tyr699) dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: signal transducer and activator of transcription 5A
• Masse moléculaire calculée: 794 aa, 92 kDa
• Poids moléculaire observé: 90-95 kDa
• Numéro d’acquisition GenBank: BC027036
• Symbole du gène: STAT5A
• Identification du gène (NCBI): 6776
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.

Publications

Species	Application	Title
mouse	WB	Carbohydr Polym; Structural characterizations and antiaging activities of hydrolyzed low-molecular-weight polysaccharides from Polygoni Multiflori Radix Praeparata; Authors - Wei Xu
	WB	J Immunother Cancer; Endosialin-positive CAFs promote hepatocellular carcinoma progression by suppressing CD8+ T cell infiltration; Authors - Lunbiao Gan
human	WB	Front Immunol; Elevated levels of exogenous prolactin promote inflammation at the maternal-fetal interface via the JAK2/STAT5B signaling axis; Authors - Alycia Williams
mouse	WB	Life Sci Alliance; Identification of TNFAIP2 as a unique cellular regulator of CSF-1 receptor activation; Authors - Randa A Abdelnaser
human	WB	Bioengineered; The long non-coding RNA T cell leukemia homeobox 1 neighbor enhances signal transducer and activator of transcription 5A phosphorylation to promote colon cancer cell invasion, migration, and metastasis.; Authors - Guanyang Chen
human,mouse	WB,IHC	Cancer Lett; FLT3-Selective PROTAC: Enhanced Safety and Increased Synergy with Venetoclax in FLT3-ITD Mutated Acute Myeloid Leukemia; Authors - Yuxin Tan

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