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Anticorps Recombinant de lapin anti-Phospho-STAT5A (Tyr694)

Phospho-STAT5A (Tyr694) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®555 Fluorescent Dye

CloneNo.

2P21

N° de cat : CL555-80115

Synonymes

MGF, STAT5, STAT5A

Galerie de données de validation

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  • Flow cytometry (FC) experiment of TF-1 cells using CoraLite®555-conjugated Phospho-STAT5A (Tyr694) Re (CL555-80115)

    FC experiment of TF-1 using CL555-80115

    1X10^6 TF-1 cells untreated (dashed lines) or treated with GM-CSF (red) were intracellularly stained with 0.03 ug CoraLite®555 Anti-Human Phospho-STAT5A (Tyr694) (CL555-80115, Clone:2P21), or 0.03 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en cytométriecellules TF-1 traitées au GM-CSF

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC)FC : 0.03 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL555-80115 cible Phospho-STAT5A (Tyr694) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet signal transducer and activator of transcription 5A
Masse moléculaire calculée 794 aa, 92 kDa
Poids moléculaire observé90-95 kDa
Numéro d’acquisition GenBankBC027036
Symbole du gène STAT5A
Identification du gène (NCBI) 6776
Conjugaison CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STAT5A, also named as STAT5, belongs to the transcription factor STAT family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5A is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different GHs. Phosphorylation at Tyr694 is obligatory for Stat5 activation. Activation of STAT5A in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of human STAT5A is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of STAT5A in cells.