Anticorps Recombinant de lapin anti-Phospho-STK11/LKB1 (Thr189)

Phospho-STK11/LKB1 (Thr189) Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5J9

N° de cat : 80127-1-PBS

Synonymes

p STK11, p STK11 (Thr189), p STK11 Thr189, p STK11/LKB1, p STK11/LKB1 (Thr189)

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Galerie de données de validation

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  WB analysis using 80127-1-RR (same clone as 80127-1-PBS)

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

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  WB analysis using 80127-1-RR (same clone as 80127-1-PBS)

  Non-treated NIH/3T3 and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

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  WB analysis using 80127-1-RR (same clone as 80127-1-PBS)

  Non-treated HEK-293 and Calyculin A treated HEK-293 cells subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

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  IF Staining of PC-3 using 80127-1-RR (same clone as 80127-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using Phospho-STK11/LKB1 (Thr189) antibody (80127-1-RR, Clone: 5J9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 80127-1-RR (same clone as 80127-1-PBS)

  1X10^6 PC-3 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.13 ug Phospho-STK11/LKB1 (Thr189) Recombinant monoclonal antibody (80127-1-RR, Clone:5J9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80127-1-PBS in a different storage buffer formulation.

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Informations sur le produit

80127-1-PBS cible Phospho-STK11/LKB1 (Thr189) dans les applications de WB, IF/ICC, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: serine/threonine kinase 11
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC007981
• Symbole du gène: LKB1
• Identification du gène (NCBI): 6794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.

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