Anticorps Recombinant de lapin anti-Phospho-STK11/LKB1 (Thr189)

Phospho-STK11/LKB1 (Thr189) Recombinant Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

CloneNo.

5J9

N° de cat : 80127-1-RR

Synonymes

LKB1, Phospho-STK11 (Thr189), PJS, serine/threonine kinase 11, STK11

---

Galerie de données de validation

• 

  WB analysis using 80127-1-RR

  Non-treated PC-3 and Calyculin A treated PC-3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 80127-1-RR

  Non-treated NIH/3T3 and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  WB analysis using 80127-1-RR

  Non-treated HEK-293 and Calyculin A treated HEK-293 cells subjected to SDS PAGE followed by western blot with 80127-1-RR (Phospho-STK11/LKB1 (Thr189) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

• 

  IF Staining of PC-3 using 80127-1-RR

  Immunofluorescent analysis of (4% PFA) fixed non-treated PC-3 cells and Calyculin A treated PC-3 cells using Phospho-STK11/LKB1 (Thr189) antibody (80127-1-RR, Clone: 5J9 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of PC-3 using 80127-1-RR

  1X10^6 PC-3 cells untreated (dashed lines) or Calyculin A treated (red) were intracellularly stained with 0.25 ug Anti-Human Phospho-STK11 (Thr189) (80127-1-RR, Clone:5J9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

---

Applications testées

• Résultats positifs en WB: cellules PC-3, cellules HEK-293, cellules HEK-293 traitées à la calyculine A, cellules NIH/3T3, cellules NIH/3T3 traitées à la calyculine A, cellules PC-3 traitées à la calyculine A
• Résultats positifs en IF: cellules PC-3 traitées à la calyculine A,
• Résultats positifs en cytométrie: cellules PC-3 traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:10000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80127-1-RR cible Phospho-STK11/LKB1 (Thr189) dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: serine/threonine kinase 11
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50-55 kDa
• Numéro d’acquisition GenBank: BC007981
• Symbole du gène: STK11
• Identification du gène (NCBI): 6794
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

STK11(serine/threonine-protein kinase 11) is also named as LKB1, PJS, and belongs to the protein kinase superfamily. It controls the activity of AMP-activated protein kinase (AMPK) family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. The tumour suppressor protein LKB1 is a serine/threonine kinase that has been causally linked to Peutz-Jeghers syndrome (PJS). Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) and defects in STK11 have been associated with testicular germ cell tumor (TGCT) and some sporadic cancers, especially lung cancers.