Anticorps Polyclonal de lapin anti-Phospho-MST1 (Thr183)/MST2 (Thr180)

Phospho-MST1 (Thr183)/MST2 (Thr180) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 28953-1-AP

Synonymes

DKFZp686A2068, KRS2, MST 1, MST1, MST1 (T183)/MST2(T180), MST2, Phospho-MST1 (T183)/MST2(T180), Phospho-MST1 (Thr183)/MST2 (Thr180), serine/threonine kinase 4, STE20 like kinase MST1, STK4, YSK3

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Galerie de données de validation

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  WB analysis using 28953-1-AP

  Non-treated Ramos and Staurosporine treated Ramos cells were subjected to SDS PAGE followed by western blot with 28953-1-AP (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:1000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: cellules Ramos traitées à la staurosporine,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 7 publications below

Informations sur le produit

28953-1-AP cible Phospho-MST1 (Thr183)/MST2 (Thr180) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: serine/threonine kinase 4
• Masse moléculaire calculée: 56 kDa
• Poids moléculaire observé: 52-56 kDa
• Numéro d’acquisition GenBank: BC005231
• Symbole du gène: MST1
• Identification du gène (NCBI): 6789
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases. MST1/2 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the MST1/2 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. MST1 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of MST1 at Thr183 (Thr180 in MST2) in the activation loop is a key activation mechanism for MST1/2 because phosphorylation of Thr183/180 causes the cleavage of MST1 by caspases under apoptotic conditions.

Publications

Species	Application	Title
human	WB	Int J Biol Sci; S100 Calcium Binding Protein A16 Promotes Cell Proliferation by triggering LATS1 ubiquitin degradation mediated by CUL4A ligase to inhibit Hippo pathway in Glioma development; Authors - Yifang Hu
rat	WB	Cell Rep; The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells; Authors - Michael R Weaver
mouse	WB	bioRxiv; The STRIPAK complex is required for radial sorting and laminin receptor expression in Schwann cells; Authors - Michael R Weaver
human	WB	Discov Oncol; Upregulated GPRC5A disrupting the Hippo pathway promotes the proliferation and migration of pancreatic cancer cells via the cAMP-CREB axis; Authors - Weidan Fang
human	WB	Cell Death Dis; PLAGL2 promotes bladder cancer progression via RACGAP1/RhoA GTPase/YAP1 signaling; Authors - Hualin Chen
human,mouse	WB	J Cell Physiol; m6 A-induced TRIB3 regulates Hippo pathway through interacting with LATS1 to promote the progression of lung adenocarcinoma; Authors - Jiamei Wu

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