Anticorps Polyclonal de lapin anti-Phospho-TAK1 (Ser439)

Phospho-TAK1 (Ser439) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 30895-1-AP

Synonymes

p TAK1, p TAK1 (Ser439), p TAK1 Ser439, Phospho TAK1, Phospho TAK1 (Ser439)

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Galerie de données de validation

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  WB analysis using 30895-1-AP

  Phosphatase inhibitor treated HEK-293T cells, λ phosphatase treated HEK-293T cells, and non-treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 30895-1-AP (Phospho-TAK1 (Ser439) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293T traitées à la λ phosphatase

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

30895-1-AP cible Phospho-TAK1 (Ser439) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase kinase kinase 7
• Masse moléculaire calculée: 579 aa, 64 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC017715
• Symbole du gène: TAK1
• Identification du gène (NCBI): 6885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. TAK1 has been identified as a crucial regulatory component of MAPK and nuclear factor-κB (NF-κB) signaling pathways, which play a key role in lipid metabolism and inflammation. Activated TAK1 then phosphorylates downstream substrates to spark the NF-κB and MAPK signaling pathways. Hence, phospho-TAK1 confers most of its regulatory functions, and inhibiting the hyperactivation of TAK1 should be a promising NASH(Nonalcoholic steatohepatitis ) therapy. (PMID: 34146477) This antibody is equivalent to Ser412 in mice.

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