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Anticorps Recombinant de lapin anti-Phospho-TAK1 (Thr187)

Phospho-TAK1 (Thr187) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

2O6

N° de cat : CL594-81785

Synonymes

MAP3K7, TAK1, Mitogen-activated protein kinase kinase kinase 7, mitogen activated protein kinase kinase kinase 7, MAP3K 7

Galerie de données de validation

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  • Flow cytometry (FC) experiment of HEK-293 cells using CoraLite®594-conjugated Phospho-TAK1 (Thr187) Reco (CL594-81785)

    FC experiment of HEK-293 using CL594-81785

    1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.25 ug Coralite®594 Phospho-TAK1 (Thr187) Recombinant Antibody (CL594-81785, Clone:2O6)(red), or 0.25 ug CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules HEK-293 traitées à la calyculine A,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-81785 cible Phospho-TAK1 (Thr187) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet mitogen-activated protein kinase kinase kinase 7
Masse moléculaire calculée 579 aa, 64 kDa
Poids moléculaire observé75-85 kDa
Numéro d’acquisition GenBankBC017715
Symbole du gène TAK1
Identification du gène (NCBI) 6885
Conjugaison CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa; 64-70 kDa and 75-80 kDa)produced by alternative splicing.

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