Anticorps Recombinant de lapin anti-Phospho-TAU (Ser404)

Phospho-TAU (Ser404) Recombinant Antibody for WB, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

5I16

N° de cat : 81383-1-PBS

Synonymes

p TAU, p TAU (Ser404), p TAU Ser404, Phospho TAU, Phospho TAU (Ser404)

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Galerie de données de validation

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  WB analysis of mouse brain using 81383-1-RR (same clone as 81383-1-PBS)

  Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 81383-1-RR (Phospho-TAU (Ser404) antibody) at dilution of 1:16000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with Beta Tubulin antibody as loading control. This data was developed using the same antibody clone with 81383-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 81383-1-RR (same clone as 81383-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded λ phosphatase treated mouse brain tissue using Phospho-TAU (Ser404) antibody (81383-1-RR, Clone: 5I16 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81383-1-PBS in a different storage buffer formulation.

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Informations sur le produit

81383-1-PBS cible Phospho-TAU (Ser404) dans les applications de WB, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: microtubule-associated protein tau
• Masse moléculaire calculée: 37-46, 79-81 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC000558
• Symbole du gène: TAU
• Identification du gène (NCBI): 4137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. The Tau protein has six isoforms produced from a single gene through alternative RNA splicing. Isoforms differ in number of inserts at the N-terminal half and the number of repeats at the C-terminal half (3 repeat-3R; 4 repeat-4R). Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

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