Anticorps Recombinant de lapin anti-Phospho-TAU (Thr205)

Phospho-TAU (Thr205) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

251173E12

N° de cat : 86669-1-RR

Synonymes

MAPT, TAU, AT180, DDPAC, MAPTL

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Galerie de données de validation

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  WB analysis using 86669-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86669-1-RR (Phospho-TAU (Thr205) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody (60004-1-Ig) as loading control. The FAD3T mouse is a triple transgenic model expressing APP, PS1, and Tau transgenes.

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  WB analysis using 86669-1-RR

  Phosphatase inhibitor treated and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 86669-1-RR (Phospho-TAU (Thr205) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody (60004-1-Ig) as loading control.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, FAD3T mouse brain tissue, λ phosphatase treated mouse brain tissue

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86669-1-RR cible Phospho-TAU (Thr205) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: microtubule-associated protein tau
• Masse moléculaire calculée: 37-46, 79-81 kDa
• Poids moléculaire observé: 45-60 kDa
• Numéro d’acquisition GenBank: BC000558
• Symbole du gène: TAU
• Identification du gène (NCBI): 4137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tau is hyperphosphorylated during aging and in age-related neurodegenerative diseases such as Alzheimer's disease (AD) and fronto-temporal dementia. Hyperphosphorylation of Tau leads to the formation of neurofibrillary tangles (NFT) in the neurons and glia cells, which is one of the hallmarks of AD.

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