Anticorps Polyclonal de lapin anti-Phospho-TAU (Thr217)

Phospho-TAU (Thr217) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 31138-1-AP

Synonymes

p Tau, p Tau (Thr217), p Tau Thr217, Phospho Tau, Phospho Tau (Thr217)

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Galerie de données de validation

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  WB analysis using 31138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31138-1-AP (Phospho-TAU (Thr217) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. The FAD3T mouse is a triple transgenic model expressing APP, PS1, and Tau transgenes.

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  WB analysis using 31138-1-AP

  Non-treated mouse brain tissue, phosphatase inhibitor treated mouse brain tissue and λ phosphatase treated mouse brain tissue were subjected to SDS PAGE followed by western blot with 31138-1-AP (Phospho-TAU (Thr217) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: FAD3T mouse brain tissue, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

31138-1-AP cible Phospho-TAU (Thr217) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: microtubule-associated protein tau
• Masse moléculaire calculée: 37-46, 79-81 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC000558
• Symbole du gène: TAU
• Identification du gène (NCBI): 4137
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Tau (tubulin-associated unit) is a microtubule-associated protein (also known as MAPT), expressed mainly in neurons of the central nervous system. Its primary function is to modulate microtubule dynamics for maintaining axonal cytoskeleton. Various isoforms of Tau exist due to the alternative splicing, and short isoforms around 45-69 kDa and long isoforms around 100-110 kDa have been reported in different literature (8752131,15965697). Phosphorylated tau at threonine 217 (p-tau217) is one of the emerging tau markers that has repeatedly shown more promise than other p-tau isoforms as a marker of AD pathology.

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