Anticorps Polyclonal de lapin anti-Phospho-TDP43 (Ser409/410)

Phospho-TDP43 (Ser409/410) Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 22309-1-PBS

Synonymes

p TDP43, p TDP43 (Ser409/410), p TDP43 Ser409/410, Phospho TDP43, Phospho TDP43 (Ser409/410)

---

Galerie de données de validation

• 

  WB analysis using 22309-1-AP (same clone as 22309-1-PBS)

  Non-treated and Calyculin A treated Neuro-2a cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22309-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 22309-1-AP (same clone as 22309-1-PBS)

  Non-treated and Calyculin A treated HeLa cellls were subjected to SDS PAGE followed by western blot with 22309-1-AP (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:1000 incubated at room temperature for 1 hours. This data was developed using the same antibody clone with 22309-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

22309-1-PBS cible Phospho-TDP43 (Ser409/410) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TAR DNA binding protein
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-50 kDa, 25-35 kDa
• Numéro d’acquisition GenBank: NM_007375
• Symbole du gène: TDP-43
• Identification du gène (NCBI): 23435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).22309-1-AP is a rabbit polyclonal antibody recognizing TDP-43 only when phosphorylated at 409/410. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.

{{ptg:RelatedPrimaryAntibodies}}