Anticorps Monoclonal anti-Phospho-TDP43 (Ser409/410)

Phospho-TDP43 (Ser409/410) Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1A2C1

N° de cat : 66318-1-PBS

Synonymes

p TDP43, p TDP43 (Ser409/410), p TDP43 Ser409/410, Phospho TDP43, Phospho TDP43 (Ser409/410)

---

Galerie de données de validation

• 

  WB analysis of HeLa using 66318-1-Ig (same clone as 66318-1-PBS)

  Untreated and EA treated HeLa cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) Antibody) at dilution of 1:850 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66318-1-Ig (same clone as 66318-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66318-1-Ig (same clone as 66318-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

• 

  WB analysis of C6 using 66318-1-Ig (same clone as 66318-1-PBS)

  C6 cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410)antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 66318-1-Ig (same clone as 66318-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 66318-1-Ig (Phospho-TDP43 (Ser409/410) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66318-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

66318-1-PBS cible Phospho-TDP43 (Ser409/410) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: TAR DNA binding protein
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 45-50 kDa, 35 kDa
• Numéro d’acquisition GenBank: NM_007375
• Symbole du gène: TDP-43
• Identification du gène (NCBI): 23435
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The TARDBP gene encodes the TDP-43 protein, initially found to repress HIV-1 transcription by binding TAR DNA. TDP-43 has since been shown to bind RNA as well as DNA, and have multiple functions in transcriptional repression, translational regulation and pre-mRNA splicing. For instance, it is reported to regulate alternate splicing of the CTFR gene. In 2006 Neumann et al. found that hyperphosphorylated, ubiquitinated and/or cleaved forms of TDP-43, collectively known as pathological TDP-43, play a major role in the disease mechanisms of ubiquitin-positive, tau- and alpha-synuclein-negative frontotemporal dementia (FTLD-U) and in amyotrophic lateral sclerosis (ALS). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176). 66318-1-Ig is a phospho-dependent monoclonal antibody specifically recognizing phospho-TDP43 (409/410). It does not react with native TDP-43.

{{ptg:RelatedPrimaryAntibodies}}