Anticorps Recombinant de lapin anti-Phospho-TSC2 (Ser939)

Phospho-TSC2 (Ser939) Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2B18

N° de cat : 81654-1-PBS

Synonymes

Phospho TSC2 (Ser939), Phospho TSC2, p TSC2 Ser939, p TSC2 (Ser939), p TSC2

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Galerie de données de validation

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  WB analysis using 81654-1-RR (same clone as 81654-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 81654-1-RR (Phospho-TSC2 (Ser939) antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with mTOR antibody (66888-1-Ig) as the loading control. This data was developed using the same antibody clone with 81654-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 81654-1-RR (same clone as 81654-1-PBS)

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A were intracellularly stained with 0.06 ug Phospho-TSC2 (Ser939) Recombinant antibody (81654-1-RR, Clone:2B18) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.06 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 81654-1-PBS in a different storage buffer formulation.

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Informations sur le produit

81654-1-PBS cible Phospho-TSC2 (Ser939) dans les applications de WB, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tuberous sclerosis 2
• Masse moléculaire calculée: 1807 aa, 201 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC150300
• Symbole du gène: TSC2
• Identification du gène (NCBI): 7249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

TSC2, also named Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. Tuberin is phosphorylated on Ser939 and Thr1462 in response to PI3K activation and the human TSC complex is a direct biochemical target of the PI3K/Akt pathway (PMID: 12150915).

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