Anticorps Polyclonal de lapin anti-Phospho-TSC2 (Thr1462)

Phospho-TSC2 (Thr1462) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(1)

Avis (1)

N° de cat : 29000-1-AP

Synonymes

FLJ43106, LAM, Phospho-TSC2 (Thr1462), TSC2, TSC4, Tuberin, tuberous sclerosis 2, Tuberous sclerosis 2 protein

---

Galerie de données de validation

• 

  WB analysis using 29000-1-AP

  Non-treated PC-3 and PDGF (HZ-1215) treated PC-3 cells were subjected to SDS PAGE followed by western blot with 29000-1-AP (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:1000 incubated at 37℃ for 1 hour.

• 

  WB analysis using 29000-1-AP

  Non-treated NIH/3T3 and PDGF (HZ-1215) treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29000-1-AP (Phospho-TSC2 (Thr1462) antibody) at dilution of 1:1000 incubated at 37℃ for 1 hour.

---

Applications testées

• Résultats positifs en WB: cellules PC-3 traitées au PDGF, cellules NIH/3T3 traitées au PDGF

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

29000-1-AP cible Phospho-TSC2 (Thr1462) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: tuberous sclerosis 2
• Masse moléculaire calculée: 1807 aa, 201 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC150300
• Symbole du gène: TSC2
• Identification du gène (NCBI): 7249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide, 50% glycerol, and 0.05% BSA
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. It has been reported that TSC2 can be phosphorylated at Thr1462 by Akt/PKB involved in stimulating cell growth and is activated by insulin. (PMID: 14651849, 12172553)

Publications

Species	Application	Title
human	WB	Research (Wash D C); The FTO Mediated N6-Methyladenosine Modification of DDIT4 Regulation with Tumorigenesis and Metastasis in Prostate Cancer; Authors - Yue Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Cali (Verified Customer) (01-21-2025)

It is okay to detect endogenous pT1462 TSC2 with the right molecular weight (about 200 kd), although some possible non-specific bands exist.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse brain lysate

Phospho-TSC2 (Thr1462) Antibody Western Blot validation (1:1000 dilution) in mouse brain lysate (Cat no:29000-1-AP)

{{ptg:RelatedPrimaryAntibodies}}