Anticorps Recombinant de lapin anti-Phospho-ULK1 (Ser758)

Phospho-ULK1 (Ser758) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240770A7

N° de cat : 82776-8-RR

Synonymes

P ULK1 (Ser758), Phos ULK1 (Ser758), Phospho-ULK1 (Ser757), Phos-ULK1 (Ser758), P-ULK1 (Ser758)

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Galerie de données de validation

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  WB analysis using 82776-8-RR

  Non-treated and IGF-1 treated MCF-7 cells were subjected to SDS PAGE followed by western blot with 82776-8-RR (Phospho-ULK1 (Ser758) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was tripped and re-blotted with Alpha Tubulin antibody (66031-1-Ig) as loading control.

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Applications testées

• Résultats positifs en WB: cellules MCF-7 traitées à l'IGF-1,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82776-8-RR cible Phospho-ULK1 (Ser758) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-51-like kinase 1 (C. elegans)
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: NM_003565
• Symbole du gène: ULK1
• Identification du gène (NCBI): 8408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367).This antibody is equivalent to Ser757 in mice.

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