Anticorps Polyclonal de lapin anti-Phospho-ULK1 (Thr468)

Phospho-ULK1 (Thr468) Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 29006-1-AP

Synonymes

ULK1, Serine/threonine-protein kinase ULK1, KIAA0722, hATG1, EC:2.7.11.1

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Galerie de données de validation

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  WB analysis using 29006-1-AP

  Non-treated HeLa, phosphatase inhibitor treated and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 29006-1-AP (Phospho-ULK1 (Thr468) antibody) at dilution of 1:4000 incubated at room temperature for 1 hours.

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  IF Staining of HeLa using 29006-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Phospho-ULK1 (Thr468) antibody (29006-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa traitées à la λ phosphatase,
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below

Informations sur le produit

29006-1-AP cible Phospho-ULK1 (Thr468) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: unc-51-like kinase 1 (C. elegans)
• Poids moléculaire observé: 130-140 kDa
• Numéro d’acquisition GenBank: NM_003565
• Symbole du gène: ULK1
• Identification du gène (NCBI): 8408
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. S467, S556, and T574 are sites responsible for regulating mitochondrial homeostasis during starvation. (PMID: 30517873, PMID: 21258367)

Publications

Species	Application	Title
pig	WB	J Nutr; Odd-chain fatty acids-enriched fats improved the growth and intestinal morphology and function in milk replacers-fed piglets; Authors - Geyan Duan
rat	WB	Brain Res Bull; N-linoleyltyrosine protects neurons against Aβ1-40-induced cell toxicity via autophagy involving the CB2/AMPK/mTOR/ULK1 pathway; Authors - Yi Zhou
human	WB	Anal Cell Pathol (Amst); Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis; Authors - Junli Zhang
human	WB	Environ Toxicol; Lysine specific demethylase 1 inhibits sodium arsenite activation of HSCs by regulating SESN2/AMPK/ULK1 signaling pathway activity; Authors - Yingwan Zhang
mouse	WB	Ecotoxicol Environ Saf; Maternal lead exposure impairs offspring growth and intestinal microbiota via AMPK/ULK1 pathway activation; Authors - Yuanyuan He

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