Anticorps Recombinant de lapin anti-Phospho-YAP1 (Ser127)

Phospho-YAP1 (Ser127) Recombinant Antibody for WB, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

240779G4

N° de cat : 80694-2-RR

Synonymes

p YAP1, p YAP1 (Ser127), p YAP1 Ser127, Phospho YAP1, Phospho YAP1 (Ser127)

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Galerie de données de validation

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  WB analysis of HepG2 using 80694-2-RR

  Calyculin A treated and untreated HepG2 cells were subjected to SDS PAGE followed by western blot with 80694-2-RR (Phospho-YAP1 (Ser127) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with GAPDH Monoclonal antibody (60004-1-Ig) as loading control.

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  FC experiment of HepG2 using 80694-2-RR

  1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A intracellularly stained with 0.13 ug Phospho-YAP1 (Ser127) Recombinant antibody (80694-2-RR, Clone:240779G4) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

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Applications testées

• Résultats positifs en WB: cellules HepG2 traitées à la calyculine A,
• Résultats positifs en FC (Intra): cellules HepG2 traitées à la calyculine A,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

80694-2-RR cible Phospho-YAP1 (Ser127) dans les applications de WB, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Yes-associated protein 1, 65kDa
• Masse moléculaire calculée: 504 aa, 54 kDa
• Poids moléculaire observé: 70-78 kDa
• Numéro d’acquisition GenBank: BC038235
• Symbole du gène: YAP1
• Identification du gène (NCBI): 10413
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

Publications

Species	Application	Title
human	WB	Colloids Surf B Biointerfaces; Decellularized human amniotic member hydrogel promotes limbal stem cells proliferation; Authors - Yongyao Tan

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