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Anticorps Recombinant de lapin anti-Phospho-YAP1 (Ser127)

Phospho-YAP1 (Ser127) Recombinant Antibody for FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

FC (Intra)

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240779G4

N° de cat : CL647-80694-2

Synonymes

Phospho YAP1, p YAP1 Ser127, p YAP1 (Ser127), p YAP1, Phospho YAP1 (Ser127)

Galerie de données de validation

Filter:
  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 647-conjugated Phospho-YAP1 (Ser127 (CL647-80694-2)

    FC experiment of HepG2 using CL647-80694-2

    1X10^6 HepG2 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Yap1 (Ser127) Recombinant Antibody (CL647-80694-2, Clone:240779G4)(red), or 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control RecAb (CL647-98136, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Applications testées

Résultats positifs en FC (Intra)cellules HepG2 traitées à la calyculine A,

Dilution recommandée

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-80694-2 cible Phospho-YAP1 (Ser127) dans les applications de FC (Intra) et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène Peptide
Nom complet Yes-associated protein 1, 65kDa
Masse moléculaire calculée 504 aa, 54 kDa
Poids moléculaire observé70-78 kDa
Numéro d’acquisition GenBankBC038235
Symbole du gène YAP1
Identification du gène (NCBI) 10413
Conjugaison CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
Conditions de stockageStocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

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