Anticorps Polyclonal de lapin anti-Phospho-YAP1 (Ser397)

Phospho-YAP1 (Ser397) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(25)

Avis (1)

N° de cat : 29018-1-AP

Synonymes

p YAP1, p YAP1 (Ser397), p YAP1 Ser397, Phospho YAP (Ser397), Phospho YAP1

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Galerie de données de validation

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  WB analysis using 29018-1-AP

  Serum-free treated NIH/3T3 cells and 10 % fetal bovine serum treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29018-1-AP (Phospho-YAP1 (Ser397) antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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Applications testées

• Résultats positifs en WB: Serum-free treated NIH/3T3 cells, cellules NIH/3T3 traitées au sérum fœtal de bovin à 10 %

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 25 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

29018-1-AP cible Phospho-YAP1 (Ser397) dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Yes-associated protein 1, 65kDa
• Masse moléculaire calculée: 504 aa, 54 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC038235
• Symbole du gène: YAP1
• Identification du gène (NCBI): 10413
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

Publications

Species	Application	Title
human	WB	Dev Cell; Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation; Authors - Wanxin Guo
human	WB	Front Immunol; The role of YAP1 in survival prediction, immune modulation, and drug response: A pan-cancer perspective; Authors - Xueqing Hu
rat,mouse	WB	Int Immunopharmacol; ASIC1a regulates ferroptosis in hepatic stellate cells via the Hippo/Yap-1 pathway in liver fibrosis; Authors - Yueqin Zhu
mouse	WB	Int Immunopharmacol; Ischemia-inhibited ferric chelate reductase 1 improves ferroptosis-mediated intestinal ischemia injury via Hippo signaling; Authors - Pengzhen Liang
human	WB	Sci Rep; Gingival proteomics reveals the role of TGF beta and YAP/TAZ signaling in Raine syndrome fibrosis; Authors - Cláudio Rodrigues Rezende Costa
human	WB	Mol Cell Biochem; RNF144A-AS1 stabilizes TAF15 and promotes malignant biological behaviors of skin cutaneous melanoma; Authors - Shude Yang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-19-2024)

Worked well to detect pYAP at 70 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

Phospho-YAP1 (Ser397) Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:29018-1-AP)

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