Anticorps Recombinant de lapin anti-Phospho-YAP1 (Ser397)

Phospho-YAP1 (Ser397) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250306F11

N° de cat : 80694-3-RR

Synonymes

p YAP1 (Ser397), Phospho YAP1 (Ser397), Phospho-YAP, Phospho-YAP (Ser397), Phospho-YAP(Ser397)

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 80694-3-RR

  NIH/3T3 cell lysates were subjected to SDS PAGE followed by western blot with 80694-3-RR (Phospho-YAP1 (Ser397) antibody) blocked with BSA only, Phospho-YAP1 (Ser397) peptide or non-Phospho peptide at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80694-3-RR cible Phospho-YAP1 (Ser397) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Yes-associated protein 1, 65kDa
• Masse moléculaire calculée: 504 aa, 54 kDa
• Poids moléculaire observé: 75 kDa
• Numéro d’acquisition GenBank: BC038235
• Symbole du gène: YAP1
• Identification du gène (NCBI): 10413
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The transcriptional factors Yes-associated protein 1 (YAP1) and PDZ-binding motif (TAZ) act as downstream effectors of the Hippo pathway, and their subcellular location and transcriptional activities are affected by multiple post-translational modifications (PTMs). After phosphorylation, YAP1/TAZ binds to the 14-3-3 protein, which induces cytoplasmic retention of YAP1/TAZ. Moreover, phosphorylated YAP1/TAZ is likely to undergo ubiquitination degradation that is dependent on the β-transducin repeat-containing E3 ubiquitin protein ligase complex. The phosphorylation of YAP at Ser127 promotes its cytoplasmic retention, whereas phosphorylation at Ser397 induces degradation. (PMID: 29682330, PMID: 26039999)

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