Anticorps Recombinant de lapin anti-Phospho-ZAP70 (Tyr319)/SYK (Tyr352)

Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

250683A9

N° de cat : 81767-2-RR

Synonymes

Phospho Syk (Tyr352), Phospho ZAP70 (Tyr319)/Syk (Tyr352), Phospho Zap-70 (Tyr319)/Syk (Tyr352), Phospho-Syk (Tyr352), Phospho-Syk-Tyr352

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Galerie de données de validation

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  WB analysis of Jurkat using 81767-2-RR

  Non-treated Jurkat cells and H2O2 treated Jurkat cells were subjected to SDS PAGE followed by western blot with 81767-2-RR (Phospho-ZAP70 (Tyr319)/Syk (Tyr352) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

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Applications testées

• Résultats positifs en WB: H2O2 treated Jurkat cells,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

81767-2-RR cible Phospho-ZAP70 (Tyr319)/SYK (Tyr352) dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: zeta-chain (TCR) associated protein kinase 70kDa
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa, 72kDa
• Numéro d’acquisition GenBank: BC039039
• Symbole du gène: ZAP70
• Identification du gène (NCBI): 7535
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. Three tyrosine residues (Tyr292, Tyr315 and Tyr319) in the interdomain B region of ZAP‐70 have attracted particular attention as sites that might regulate ZAP‐70 activity and/or binding to heterologous proteins. The third candidate phosphorylation site in the interdomain B region of ZAP‐70 is Tyr319. Although the functional importance of this site in ZAP‐70 is unknown, a previous report presented indirect evidence that the orthologous Tyr352 residue in Syk is involved in the association of PLC‐γ1 with this PTK. (PMID: 10202147)

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