Anticorps Polyclonal de lapin anti-Phospho-c-MYC (Ser62)

Phospho-c-MYC (Ser62) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28915-1-PBS

Synonymes

c-MYC (Ser62), p c-MYC, p c-MYC (Ser62), p c-MYC Ser62, p-c-MYC

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Galerie de données de validation

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  WB analysis using 28915-1-AP (same clone as 28915-1-PBS)

  Non-treated and MG132 treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28915-1-AP (Phospho-c-MYC (Ser62) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28915-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 28915-1-AP (same clone as 28915-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-c-MYC (Ser62) antibody (28915-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28915-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28915-1-PBS cible Phospho-c-MYC (Ser62) dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: v-myc myelocytomatosis viral oncogene homolog (avian)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC000141
• Symbole du gène: MYC
• Identification du gène (NCBI): 4609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MYC is a transcription factor that globally enhances expression of transcribing genes, including those vital for cell cycle, growth, proliferation, and survival in normal and cancer cells. MYC has multiple isomers, and this antibody recognizes the phosphorylation of the 439 amino acid isomer (P01106-1, UniProt) at the 62 serine site. Posttranslational modifications that regulate MYC stability include phosphorylations at Ser62 and Thr58. Phosphorylation at Ser62, primarily by ERK, stabilizes MYC. While kinases other than ERK phosphorylate Ser62, GSK3β has been the only kinase known to phosphorylate MYC at Thr58.(PMID: 32482868)

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