Anticorps Recombinant de lapin anti-Phospho-eEF2K (Ser366)

Phospho-eEF2K (Ser366) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250361E10

N° de cat : 80351-3-PBS

Synonymes

eEF2K (Ser366), eEF2K(Ser366), p eEF2K, p eEF2K (Ser366), p-eEF2K

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Galerie de données de validation

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  WB analysis of Jurkat using 80351-3-RR (same clone as 80351-3-PBS)

  Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 80351-3-RR (Phospho-eEF2K (Ser366) antibody) blocked with BSA only, Phospho-eEF2K (Ser366) peptide or non-Phospho peptide at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80351-3-PBS in a different storage buffer formulation.

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Informations sur le produit

80351-3-PBS cible Phospho-eEF2K (Ser366) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic elongation factor-2 kinase
• Masse moléculaire calculée: 725 aa, 100 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC032665
• Symbole du gène: EEF2K
• Identification du gène (NCBI): 29904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic Elongation Factor-2 Kinase (eEF2K) acts as a negative regulator of protein synthesis, translation, and cell growth. As a structurally unique member of the alpha-kinase family, eEF2K is essential to cell survival under stressful conditions, as it contributes to both cell viability and proliferation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is regulated by various mechanisms, including phosphorylation through residues and autophosphorylation. eEF2K is downregulated through the phosphorylation of multiple sites via mTOR signaling and upregulated via the AMPK pathway. In the S6K-mediated pathway, RPS6KB1 or p70S6K, can phosphorylate eEF2K on Ser-366, rendering it inactive(PMID: 11500364, PMID: 34532346). The calculated molecular weight of eEF2K is 82kDa, and probably due to phosphorylation modification, resulting in a larger molecular weight, around 100kDa.

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