Anticorps Recombinant de lapin anti-Phospho-eEF2 (Thr57)

Phospho-eEF2 (Thr57) Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250222A6

N° de cat : 80351-2-PBS

Synonymes

p eEF2, p eEF2 (Thr57), p-eEF2, p-eEF2 (Thr57), p-eEF2(Thr57)

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Galerie de données de validation

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  WB analysis using 80351-2-RR (same clone as 80351-2-PBS)

  HeLa cell lysates were subjected to SDS PAGE followed by western blot with 80351-2-RR (Phospho-eEF2K (Thr57) antibody) blocked with BSA only, Phospho-eEF2K (Thr57) peptide or non-Phospho peptide at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80351-2-PBS in a different storage buffer formulation.

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Informations sur le produit

80351-2-PBS cible Phospho-eEF2 (Thr57) dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: eukaryotic translation elongation factor 2
• Masse moléculaire calculée: 858 aa, 95 kDa
• Poids moléculaire observé: 100 kDa
• Numéro d’acquisition GenBank: BC126259
• Symbole du gène: EEF2
• Identification du gène (NCBI): 29904
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

eEF2 (Eukaryotic Elongation Factor 2) is a key component in the process of protein synthesis in eukaryotic cells. It is a GTP-binding protein that plays a crucial role in the elongation phase of translation. Specifically, eEF2 facilitates the movement of the ribosome along the mRNA, allowing the addition of amino acids to the growing polypeptide chain. This elongation factor is essential for efficient and accurate protein synthesis. Interestingly, eEF2 phosphorylation can either be increased or decreased depending on the stimulus used in learning or synaptic plasticity paradigms. Examining the specific contributions of increases and decreases in eEF2 phosphorylation to learning and memory is difficult. (PMID: 25762683)

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