Anticorps Monoclonal anti-Phospho-mTOR (Ser2448)

Phospho-mTOR (Ser2448) Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

2A12G3

N° de cat : 67778-1-PBS

Synonymes

mTOR (Ser2448), p mTOR, p mTOR (Ser2448), p mTOR Ser2448, Phospho mTOR

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Galerie de données de validation

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  WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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  WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

  Non-treated and EGF treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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  WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

  Non-treated and Rapamycin treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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  WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

  Non-treated and Calyculin A treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67778-1-Ig (same clone as 67778-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67778-1-Ig (same clone as 67778-1-PBS)

  Non-treated and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 67778-1-Ig (Phospho-mTOR (Ser2448) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with RICTOR antibody (66867-2-Ig) and mTOR antibody (66888-1-Ig) subsequently. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67778-1-Ig (same clone as 67778-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-mTOR (Ser2448) antibody (67778-1-Ig, Clone: 2A12G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67778-1-Ig (same clone as 67778-1-PBS)

  1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.5 ug Anti-Human Phospho-mTOR (Ser2448) (67778-1-Ig, Clone:2A12G3) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 67778-1-Ig (same clone as 67778-1-PBS)

  1x10^6 100 nM Calyculin A (30 minutes) treated HeLa cells were intracellularly stained with 0.2 μg Phospho-mTOR (Ser2448) Monoclonal antibody (67778-1-Ig, Clone:2A12G3) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005), and 0.2 μg KLH (66360-3-Ig, Clone: K11B8C4B5). Cells were fixed with 4% PFA . This data was developed using the same antibody clone with 67778-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67778-1-PBS cible Phospho-mTOR (Ser2448) dans les applications de WB, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: FK506 binding protein 12-rapamycin associated protein 1
• Masse moléculaire calculée: 289 kDa
• Poids moléculaire observé: 289 kDa
• Numéro d’acquisition GenBank: BC117166
• Symbole du gène: mTOR
• Identification du gène (NCBI): 2475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

MTOR, also named as FRAP1, FRAP, FRAP2 and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

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