Anticorps Polyclonal de lapin anti-Phospho-mTOR (Ser2481)

Phospho-mTOR (Ser2481) Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (2)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 28879-1-AP

Synonymes

FLJ44809, FRAP, FRAP1, FRAP2, Mammalian target of rapamycin, MTOR, Phospho-mTOR (Ser2481), RAFT1, Rapamycin target protein 1, RAPT1

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Galerie de données de validation

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  WB analysis using 28879-1-AP

  Nontreated and insulin-treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28879-1-AP (Phospho-mTOR (Ser2481) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3 traitées à l'insuline

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 11 publications below

Informations sur le produit

28879-1-AP cible Phospho-mTOR (Ser2481) dans les applications de WB, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: FK506 binding protein 12-rapamycin associated protein 1
• Masse moléculaire calculée: 289 kDa
• Poids moléculaire observé: 250-289 kDa
• Numéro d’acquisition GenBank: BC117166
• Symbole du gène: mTOR
• Identification du gène (NCBI): 2475
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTOR, also named FRAP1, FRAP, FRAP2, and RAPT1, belongs to the PI3/PI4-kinase family. MTOR is a Ser/Thr protein kinase that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth. MTOR is the kinase subunit of both mTORC1 and mTORC2, which regulate cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino acids. mTORC2 is also activated by growth factors but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress fibers or F-actin. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481. mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors.

Publications

Species	Application	Title
human	WB	Anal Cell Pathol (Amst); Shikonin Induces Autophagy and Apoptosis in Esophageal Cancer EC9706 Cells by Regulating the AMPK/mTOR/ULK Axis; Authors - Junli Zhang
rat	WB	Am J Transl Res; Integrating network pharmacology with experimental validation to investigate the mechanism of Wuwei Zishen formula in improving perimenopausal syndrome; Authors - Xuewen Fu
human	WB	Naunyn Schmiedebergs Arch Pharmacol; Network pharmacology-based strategy to reveal the mechanism of pinocembrin against ovarian cancer; Authors - Guanghui Wang
human	WB	Sci Rep; Morphine promotes non-small cell lung cancer progression by downregulating E-cadherin via the PI3K/AKT/mTOR pathway; Authors - Fulei Gu
mouse	WB	Int J Biol Macromol; Lactoferrin ameliorates cognitive impairment in D-galactose-induced aging mice by regulating the PI3K/Akt/mTOR signaling pathway and the microbiome-gut-brain axis; Authors - Mengqi Wang
mouse	WB	Eur J Neurosci; Oleracein E Rejuvenates Senescent Hippocampal NSCs by Inhibiting the ERK1/2-mTOR Axis to Improve Cognitive Dysfunction in Vascular Dementia; Authors - Haili Lang

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