Anticorps Polyclonal de lapin anti-Phospho-p38 MAPK (Thr180/Tyr182)

Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(267)

N° de cat : 28796-1-AP

Synonymes

p p38 MAPK, p p38 MAPK (Thr180/Tyr182), p38 alpha/MAPK14 (Thr180/Tyr182), p38 MAPK (Thr180/Tyr182), Phospho p38 MAPK

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Galerie de données de validation

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  WB analysis using 28796-1-AP

  Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  WB analysis using 28796-1-AP

  Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28796-1-AP

  Non-treated NIH/3T3 cells and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28796-1-AP

  Non-treated C6 cells and PMA treated C6 cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28796-1-AP

  Non-treated PC-12 cells and BDNF treated PC-12 cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IF Staining of THP-1 using 28796-1-AP

  Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 28796-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HepG2 using 28796-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T traitées au UV, cellules NIH/3T3 traitées au UV, cellules THP-1 traitées au LPS
• Résultats positifs en IF/ICC: cellules THP-1 traitées au LPS, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 261 publications below
• IHC: See 8 publications below
• IF: See 7 publications below

Informations sur le produit

28796-1-AP cible Phospho-p38 MAPK (Thr180/Tyr182) dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 14
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC031574
• Symbole du gène: p38 MAPK
• Identification du gène (NCBI): 1432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)

Publications

Species	Application	Title
mouse	WB	Brain Behav Immun; Egln3 expression in microglia enhances the neuroinflammatory responses in Alzheimer's disease; Authors - Jiaxin Guan
	WB	Sci Adv; Asparagine transporter supports macrophage inflammation via histone phosphorylation; Authors - Chuanlong Wang
mouse	WB	Redox Biol; Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice; Authors - Boying Zhao
mouse	WB	EMBO Mol Med; Targeting HPK1 inhibits neutrophil responses to mitigate post-stroke lung and cerebral injuries; Authors - Tingting Zhang
mouse	WB	Clin Transl Med; PINK1 modulates Prdx2 to reduce lipotoxicity-induced apoptosis and attenuate cardiac dysfunction in heart failure mice with a preserved ejection fraction; Authors - Hao Zhang
rat	WB	J Neuroinflammation; H3K18 lactylation-mediated nucleotide-binding oligomerization domain-2 (NOD2) expression promotes bilirubin-induced pyroptosis of astrocytes; Authors - Jing Li

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