Anticorps Polyclonal de lapin anti-Phospho-p38 MAPK (Thr180/Tyr182)

Phospho-p38 MAPK (Thr180/Tyr182) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28796-1-PBS

Synonymes

MAPK14, p38 MAPK, Cytokine suppressive anti-inflammatory drug-binding protein, CSPB1, CSBP2

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Galerie de données de validation

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  WB analysis using 28796-1-AP (same clone as 28796-1-PBS)

  Non-treated and UV treated HEK-293T cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:2000 incubated at room temperature for 1 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

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  WB analysis using 28796-1-AP (same clone as 28796-1-PBS)

  Non-treated and LPS treated THP-1 cells were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

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  WB analysis using 28796-1-AP (same clone as 28796-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28796-1-AP (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

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  IF Staining of THP-1 using 28796-1-AP (same clone as 28796-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed LPS treated THP-1 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28796-1-AP (same clone as 28796-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 28796-1-AP (same clone as 28796-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Phospho-p38 MAPK (Thr180/Tyr182) antibody (28796-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 28796-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28796-1-PBS cible Phospho-p38 MAPK (Thr180/Tyr182) dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 14
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC031574
• Symbole du gène: p38 MAPK
• Identification du gène (NCBI): 1432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)

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