Anticorps Recombinant de lapin anti-Phospho-p38 MAPK (Thr180/Tyr182)

Phospho-p38 MAPK (Thr180/Tyr182) Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242308D3

N° de cat : 81212-2-PBS

Synonymes

p38 (Thr180/Tyr182), p38 MAPK (Thr180/Tyr182), phospho-p38, phospho-p38 (Thr180/Tyr182), Phospho-p38 MAPK

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Galerie de données de validation

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  WB analysis using 81212-2-RR (same clone as 81212-2-PBS)

  Non-treated NIH/3T3 cells, UV treated and Anisomycin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 81212-2-RR (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81212-2-PBS in a different storage buffer formulation.

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  WB analysis using 81212-2-RR (same clone as 81212-2-PBS)

  Non-treated HeLa cells and Anisomycin treated HeLa cells were subjected to SDS PAGE followed by western blot with 81212-2-RR (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 81212-2-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 81212-2-RR (same clone as 81212-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 81212-2-RR (Phospho-p38 MAPK (Thr180/Tyr182) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81212-2-PBS in a different storage buffer formulation.

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Informations sur le produit

81212-2-PBS cible Phospho-p38 MAPK (Thr180/Tyr182) dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: mitogen-activated protein kinase 14
• Masse moléculaire calculée: 360 aa, 41 kDa
• Poids moléculaire observé: 38-42 kDa
• Numéro d’acquisition GenBank: BC031574
• Symbole du gène: p38 MAPK
• Identification du gène (NCBI): 1432
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

A stress-activated serine/threonine protein kinase, p38 mitogen-activated protein kinase (p38 MAPK), belongs to the MAP kinase superfamily. Diverse extracellular stimuli, including ultraviolet light, irradiation, heat shock, high osmotic stress, proinflammatory cytokines and certain mitogens, trigger a stress-regulated protein kinase cascade culminating in activation of p38 MAPK through phosphorylation on a TGY motif within the kinase activation loop. The p38 MAPK undergoes dual phosphorylation at Thr182 and Tyr180 in the Thr-Gly-Tyr activation loop by MAP kinase kinase 6 (MKK6). Upon activation, p38 MAPK phosphorylates multiple substrates, including MAPK activated protein kinase 2 (MAPKAPK2) and activating transcription factor 2 (ATF-2). (PMID: 26901653, PMID: 10807318)

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