Anticorps Polyclonal de lapin anti-Phospho-p70(S6K) (Thr421/Ser424)

Phospho-p70(S6K) (Thr421/Ser424) Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 29248-1-AP

Synonymes

p p70(S6K), p p70(S6K) (Thr421/Ser424), p p70(S6K) Thr421/Ser424, p70 S6 Kinase (Thr421/Ser424), p70(S6K) (Thr421/Ser424)

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Galerie de données de validation

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  WB analysis using 29248-1-AP

  Non-treated MCF-7 cells, phosphatase inhibitor treated and λ phosphatase MCF-7 cells were subjected to SDS PAGE followed by western blot with 29248-1-AP (Phospho-p70(S6K) (Thr421/Ser424) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.

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  IF Staining of HeLa using 29248-1-AP

  Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated HeLa cells using Phospho-p70(S6K) (Thr421/Ser424) antibody (29248-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: λ phosphatase treated MCF-7 cells,
• Résultats positifs en IF/ICC: cellules HeLa traitées à la λ phosphatase,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

29248-1-AP cible Phospho-p70(S6K) (Thr421/Ser424) dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: ribosomal protein S6 kinase, 70kDa, polypeptide 1
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 65-85 kDa
• Numéro d’acquisition GenBank: BC053365
• Symbole du gène: p70 S6K
• Identification du gène (NCBI): 6198
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K), which is a serine/threonine kinase regulated by phosphoinositide 3-kinase (PI3K)/mammalian target of rapamycin (mTOR) pathway. P70S6K plays a crucial role in controlling cell cycle, growth and survival. The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation and metabolism and is the central regulator of translation of some components of protein synthesis system. Due to alternative translation two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). In the conventional model, Thr421/Ser424 sites are phosphorylated before Thr389 phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) and subsequent Thr229 phosphorylation by PDK1. (PMID: 25100792, PMID: 24970012, PMID: 21602892) The antibody also recognizes the phosphorylation of p85(S6K) at Thr444/Ser427.

Publications

Species	Application	Title
human	WB	Metabolism; Anti-sarcopenic effects of active vitamin D through modulation of anabolic and catabolic signaling pathways in human skeletal muscle: A randomized controlled trial; Authors - Tetsuya Kawahara

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