Anticorps Recombinant de lapin anti-Piezo1 (extracellular domain)

Piezo1 (extracellular domain) Recombinant Antibody for IF/ICC

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4B15

N° de cat : CL488-82625-4

Synonymes

FAM38A, PIEZO1, 4B15, KIAA0233, Membrane protein induced by beta-amyloid treatment

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Galerie de données de validation

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  IF Staining of THP-1 using CL488-82625-4

  Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite® Plus 488 Piezo1 (extracellular domain) antibody (CL488-82625-4, Clone: 4B15 ) at dilution of 1:200.

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Applications testées

• Résultats positifs en IF/ICC: cellules THP-1,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-82625-4 cible Piezo1 (extracellular domain) dans les applications de IF/ICC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Piezo1 (extracellular domain) Protéine recombinante Ag7791
• Nom complet: family with sequence similarity 38, member A
• Masse moléculaire calculée: 286 kDa
• Poids moléculaire observé: 233-286 kDa
• Numéro d’acquisition GenBank: BC008073
• Symbole du gène: Piezo1/FAM38A
• Identification du gène (NCBI): 9780
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mechanotransduction, the conversion of mechanical force into biological signals, is a fundamental physiologic process of mammalian cells that influences many critical processes including embryonic development, tactile, pain, and auditory sensation, regulation of vascular tone, flow sensing in the kidney, and muscle and tendon stretch. FAM38A, also known as Piezo1, has recently been identified as a mechanotransduction protein that gets involved in mechanosensation and stretch-activated cation channel activation. Piezo1 also plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER. Mutations in the gene encoding Piezo1 are associated with hereditary xerocytosis. Piezo1 also regulates extrusion to maintain homeostatic epithelial cell numbers. This antibody was raised against the extracellular domain of human Piezo1. (PMID: 20016066, 28416610)

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