Anticorps Recombinant de lapin anti-Plakophilin 1

Plakophilin 1 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242834G8

N° de cat : 85217-4-RR

Synonymes

PKP1, 242834G8, B6P, Plakophilin-1

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Galerie de données de validation

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  WB analysis using 85217-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85217-4-RR (Plakophilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 85217-4-RR

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 85217-4-RR (Plakophilin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85217-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85217-4-RR against Human Plakophilin 1 were performed. The affinity constant is 11.4 nM.

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Applications testées

• Résultats positifs en WB: tissu cutané de souris, cellules A431, cellules HEK-293, cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85217-4-RR cible Plakophilin 1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Plakophilin 1 Protéine recombinante Ag18153
• Nom complet: plakophilin 1 (ectodermal dysplasia/skin fragility syndrome)
• Masse moléculaire calculée: 747 aa, 83 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC114571
• Symbole du gène: PKP1
• Identification du gène (NCBI): 5317
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Plakophilin 1, also known as PKP1, B6P, is a member of the arm-repeat protein family. PKP1 contains arm-repeat (armadillo) domains, localizes to nuclei and cell desmosomes, and participates in linking cadherins to intermediate filaments in the cytoskeleton (PMID: 10747098). PKP1 contributes to epidermal morphogenesis and facilitates the formation of intermediate filaments. Mutations in PKP1 have been associated with the ectodermal dysplasia/skin fragility syndrome (PubMed:9326952, 10852826).

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