Anticorps Polyclonal de lapin anti-Plexin B2

• Phare
• Validé par KD/KO

Plexin B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 10602-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of A549 using 10602-1-AP (same clone as 10602-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 10602-1-AP (same clone as 10602-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse ovary using 10602-1-AP (same clone as 10602-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 10602-1-AP (same clone as 10602-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10602-1-AP (same clone as 10602-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 10602-1-AP (same clone as 10602-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 10602-1-AP (same clone as 10602-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10602-1-AP (same clone as 10602-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 10602-1-AP (same clone as 10602-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10602-1-AP (Plexin B2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 10602-1-AP (same clone as 10602-1-PBS)

  IP result of anti-Plexin B2 (IP:10602-1-AP, 4ug; Detection:10602-1-AP 1:1000) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 10602-1-AP (same clone as 10602-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10602-1-AP (Plexin B2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 10602-1-AP (same clone as 10602-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 10602-1-AP (Plexin B2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10602-1-PBS in a different storage buffer formulation.

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Informations sur le produit

10602-1-PBS cible Plexin B2 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Plexin B2 Protéine recombinante Ag0941
• Nom complet: plexin B2
• Masse moléculaire calculée: 205 kDa
• Poids moléculaire observé: 80 kDa, 240 kDa
• Numéro d’acquisition GenBank: BC004542
• Symbole du gène: PLXNB2
• Identification du gène (NCBI): 23654
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Semaphorins, originally identified as axon guidance molecules, have also been implicated in angiogenesis, immunoregulation, and cancer (PMID: 16584533). Plexins are main receptors for semaphorins and are thought to control many of the functional effects of semaphorins. PLXNB2 is one of the three plexin-B family members which participate in axon guidance and cell migration in response to semaphorins. It has been reported that plexin B2 precursor is 240 kDa and can be cleaved into alpha subunit (~170-kDa) and beta subunit (80-kDa) (PMID: 12533544). This antibody raised against 1542-1841aa of human plexin B2 can recognize precursor and beta subunit of plexin B2.

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