Anticorps Monoclonal anti-PLVAP

PLVAP Monoclonal Antibody for FC

Hôte / Isotype

Rat / IgG2a, kappa

Réactivité testée

souris

Applications

FC

Conjugaison

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

MECA-32

N° de cat : CL647-65214

Synonymes

MECA32, MECA-32, Panendothelial cell antigen, Plasmalemma vesicle protein 1, PV 1

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Galerie de données de validation

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  FC experiment of bEnd.3 using CL647-65214

  1x10^6 bEnd.3 cells were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse PLVAP (MECA-32) (CL647-65214, Clone:MECA-32) (red) or 0.25 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules bEnd.3,

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL647-65214 cible PLVAP dans les applications de FC et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Hôte / Isotype: Rat / IgG2a, kappa
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Mouse lymph node stromal cells
• Nom complet: plasmalemma vesicle associated protein
• Numéro d’acquisition GenBank: BC013517
• Symbole du gène: Plvap
• Identification du gène (NCBI): 84094
• Conjugaison: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.09% sodium azide and 0.5% BSA
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

Plasmalemma vesicle-associated protein (PLVAP), also known as PV-1, PAL-E and MECA-32 antigen, is an endothelial cell-specific type II membrane N-glycosylated glycoprotein with a molecular weight of approximately 50-65 kDa, that forms homodimers in situ (PMID: 7626790; 30231925). The MECA-32 antibody, derived from rats immunized with murine lymph node stroma, shows high specificity for mouse endothelium in both embryonic and mature tissues (PMID: 2683264; 7626790). It recognizes most endothelial cells in the embryonic and in the adult mouse, with the exception of the brain, skeletal, and cardiac muscle, where the MECA-32 antigen has a more restricted distribution. In skeletal and cardiac muscle only small arterioles and venules express the MECA-32 antigen, while in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier (PMID: 7626790).

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