Anticorps Monoclonal anti-Podocalyxin

Podocalyxin Monoclonal Antibody for IHC, IF-P, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

4A3C7

N° de cat : 68250-1-PBS

Synonymes

PODXL, 4A3C7, GCTM 2 antigen, GCTM-2 antigen, Gp200

---

Galerie de données de validation

• 

  IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 68250-1-Ig (Podocalyxin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human kidney using 68250-1-Ig (same clone as 68250-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human kidney tissue using Podocalyxin antibody (68250-1-Ig, Clone: 4A3C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68250-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

68250-1-PBS cible Podocalyxin dans les applications de IHC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Podocalyxin Protéine recombinante Ag12859
• Nom complet: podocalyxin-like
• Masse moléculaire calculée: 526 aa, 55 kDa
• Poids moléculaire observé: 60-70 kDa
• Numéro d’acquisition GenBank: BC093730
• Symbole du gène: Podocalyxin
• Identification du gène (NCBI): 5420
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Podocalyxin, also known as podocalyxin-like protein 1 (PODXL or PCLP1), is a transmembrane glycoprotein belonging to the CD34 family of sialomucins. Podocalyxin was originally identified as the major sialoprotein on podocytes of the kidney glomerulus, but was later found to be expressed on vascular endothelial cells and early hematopoietic progenitors. It Involved in the regulation of both adhesion and cell morphology. In addition, podocalyxin is highly expressed in embryonic stem cells and aberrant expression of podocalyxin has been implicated in a wide range of cancers. Podocalyxin is a protein with a peptide bone of ∼55.5 kDa that undergoes a post‐translational glycosylation, the different molecular mass of podocalyxin indicates the extent and variability of glycosylation patterns (PMID: 17092254).

{{ptg:RelatedPrimaryAntibodies}}