Anticorps Monoclonal anti-Podoplanin

Podoplanin Monoclonal Antibody for IHC, IF-P, FC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

IHC, IF-P, FC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D9F3

N° de cat : 67432-1-PBS

Synonymes

PDPN, 1D9F3, 29kDa cytosolic podoplanin intracellular domain, Aggrus, PA2.26 antigen

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Galerie de données de validation

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  IHC staining of human tonsillitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (PDPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 67432-1-Ig (Podoplanin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse small intestine using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse small intestine tissue using Podoplanin antibody (67432-1-Ig, Clone: 1D9F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  IF Staining of A-253 using 67432-1-Ig (same clone as 67432-1-PBS)

  Immunofluorescent analysis of un-fixed A-253 cells using Podoplanin antibody (67432-1-Ig, Clone: 1D9F3 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  FC experiment of MG-63 using 67432-1-Ig (same clone as 67432-1-PBS)

  1x10^6 MG-63 cells were surface stained with 0.2 μg Podoplanin Monoclonal antibody (67432-1-Ig, Clone:1D9F3) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005), and 0.2 μg KLH (66360-2-Ig, Clone: K11A1B2A2). Cells were not fixed. This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  FC experiment of A-253 using 67432-1-Ig (same clone as 67432-1-PBS)

  1x10^6 A-253 cells were surface stained with 0.2 μg Podoplanin Monoclonal antibody (67432-1-Ig, Clone:1D9F3) and Multi-rAb CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(RGAM005), Mouse IgG2a isotype control (66360-2-Ig, Clone: 11A1B2) was parallel stained as control. Cells were not fixed. This data was developed using the same antibody clone with 67432-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50340-1

  Cytometric bead array standard curve of MP50340-1, PDPN Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67432-2-PBS. Detection antibody: 67432-1-PBS. Standard:Ag17691. Range: 1.563-100 ng/mL.

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Informations sur le produit

67432-1-PBS cible Podoplanin dans les applications de IHC, IF-P, FC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Podoplanin Protéine recombinante Ag17691
• Nom complet: podoplanin
• Masse moléculaire calculée: 238 aa, 25 kDa
• Numéro d’acquisition GenBank: BC022812
• Symbole du gène: Podoplanin
• Identification du gène (NCBI): 10630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Podoplanin was identified as a glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.

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