Anticorps Recombinant de lapin anti-Podoplanin

Podoplanin Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

251406B11

N° de cat : 86750-2-RR

Synonymes

PDPN, 29kDa cytosolic podoplanin intracellular domain, Aggrus, PA2.26 antigen, PSEC0003

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Galerie de données de validation

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  WB analysis using 86750-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86750-2-RR (PDPN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsil using 86750-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86750-2-RR (PDPN antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsil using 86750-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsil tissue slide using 86750-2-RR (PDPN antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendix using 86750-2-RR

  Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 86750-2-RR (PDPN antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human appendix using 86750-2-RR

  Immunohistochemical analysis of paraffin-embedded human appendix tissue slide using 86750-2-RR (PDPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 86750-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86750-2-RR (PDPN antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 86750-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 86750-2-RR (PDPN antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HEK-293, tissu placentaire humain
• Résultats positifs en IHC: tissu amygdalien humain, tissu d'appendice humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

86750-2-RR cible Podoplanin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Podoplanin Protéine recombinante Eg5024
• Nom complet: podoplanin
• Poids moléculaire observé: 36-43 kDa
• Numéro d’acquisition GenBank: BC014668
• Symbole du gène: Podoplanin
• Identification du gène (NCBI): 10630
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Podoplanin was identified as a 43 kDa glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.

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