Anticorps Monoclonal anti-Podoplanin

Podoplanin Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

FC

Conjugaison

CoraLite®594 Fluorescent Dye

CloneNo.

1D9F3

N° de cat : CL594-67432

Synonymes

PDPN, 1D9F3, 29kDa cytosolic podoplanin intracellular domain, Aggrus, PA2.26 antigen

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Galerie de données de validation

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  FC experiment of U2OS using CL594-67432

  1x10^6 U2OS cells were surface stained with 0.4 ug Coralite®594 Podoplanin Monoclonal Antibody (CL594-67432, Clone:1D9F3)(red), or 0.4 ug CoraLite®594 Mouse IgG2a isotype control (11A1B2) (CL594-66360-2, Clone: 11A1B2) (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en cytométrie: cellules U2OS,

Dilution recommandée

• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL594-67432 cible Podoplanin dans les applications de FC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Podoplanin Protéine recombinante Ag17691
• Nom complet: podoplanin
• Masse moléculaire calculée: 238 aa, 25 kDa
• Numéro d’acquisition GenBank: BC022812
• Symbole du gène: Podoplanin
• Identification du gène (NCBI): 10630
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Podoplanin was identified as a glycoprotein found in the cell membranes of glomerular epithelial cells (podocyte) (PMID: 9327748). It is a lymphatic marker because the expression of podoplanin has been detected in lymphatic but not blood vascular endothelium, and is useful as the marker of tumor-associated Lymphangiogenesis. Podoplanin has a function in developing testis, most likely at the level of cell-cell interactions among pre-meiotic germ cells and immature Sertoli cells. It may be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, PDPN induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protrusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. It is required for normal lung cell proliferation and alveolus formation at birth. PDPN induces platelet aggregation. It does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels. The antibody is specific to Podoplanin.

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