Anticorps Polyclonal de lapin anti-Polycystin 2

Polycystin 2 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

canin, Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19126-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 19126-1-AP (same clone as 19126-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 19126-1-AP (same clone as 19126-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

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  WB analysis of human kidney using 19126-1-AP (same clone as 19126-1-PBS)

  human kidney tissue were subjected to SDS PAGE followed by western blot with 19126-1-AP (Polycystin 2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse testis using 19126-1-AP (same clone as 19126-1-PBS)

  IP result of anti-Polycystin 2 (IP:19126-1-AP, 4ug; Detection:19126-1-AP 1:500) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 19126-1-AP (same clone as 19126-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19126-1-AP (Polycystin 2 antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 19126-1-AP (same clone as 19126-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 19126-1-AP (Polycystin 2 antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 19126-1-AP (same clone as 19126-1-PBS)

  Immunofluorescent analysis of MDCK cells using 19126-1-AP (Polycystin 2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19126-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19126-1-PBS cible Polycystin 2 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons canin, Humain, rat, souris

• Réactivité: canin, Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: polycystic kidney disease 2 (autosomal dominant)
• Masse moléculaire calculée: 110 kDa
• Poids moléculaire observé: 109 kDa
• Numéro d’acquisition GenBank: NM_000297
• Symbole du gène: PKD2
• Identification du gène (NCBI): 5311
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Polycystin 2 (PKD2), the product of the gene mutated in type 2 autosomal dominant polycystic kidney disease, belongs to the polycystin family. PKD2 is a ~110-kDa six-transmembrane channel protein with cytoplasmic N- and C-termini. This protein functions as a Ca2+-activated intracellular Ca2+ release channel in the endoplasmic reticulum. It is also present in the plasma membrane, where it functions as a nonselective cation channel. In addition, PKD2 expression has been documented in the primary cilium of kidney epithelial cells, where it is believed to have an essential role in mediating Ca2+ entry in response to flow rate changes, suggesting that it may be part of a mechanosensing machinery residing in the primary cilium. (PMID: 16135816; 10497221)

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