Anticorps Recombinant de lapin anti-PON2

PON2 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

242673H1

N° de cat : 85126-4-PBS

Synonymes

242673H1, A-esterase 2, Aromatic esterase 2, EC:3.1.1.81, PON 2

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Galerie de données de validation

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  WB analysis using 85126-4-RR (same clone as 85126-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85126-4-PBS in a different storage buffer formulation.

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  WB analysis using 85126-4-RR (same clone as 85126-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85126-4-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01843-1

  Sandwich ELISA standard curve of MP01843-1, Mouse Pon2 Recombinant Matched Antibody Pair - PBS only. 85126-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2741. 85126-2-PBS was HRP conjugated as the detection antibody. Range: 0.391-25 ng/mL.

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  Sample test of MP01843-1

  The mean Pon2 concentration was determined to be 344.03 ng/mL in mouse liver tissue extract based on a 3.70 mg/mL extract load, 812.45 ng/mL in rat liver tissue extract based on a 2.60 mg/mL extract load and 19.36 ng/mL in NIH/3T3 cell extract based on a 1.50 mg/mL extract load.

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  Cytometric bead array standard curve of MP01843-1

  Cytometric bead array standard curve of MP01843-1, MOUSE Pon2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85126-4-PBS. Detection antibody: 85126-2-PBS. Standard: Eg2741. Range: 1.563-200 ng/mL.

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Informations sur le produit

85126-4-PBS cible PON2 dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PON2 Protéine recombinante Eg2741
• Nom complet: paraoxonase 2
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: NM_183308.2
• Symbole du gène: Pon2
• Identification du gène (NCBI): 330260
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PON2 (Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).

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