Anticorps Recombinant de lapin anti-Pon3

Pon3 Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

251535H2

N° de cat : 86577-1-PBS

Synonymes

EC:3.1.8.1, Paraoxanase-3, Serum paraoxonase/lactonase 3

---

Galerie de données de validation

• 

  WB analysis using 86577-1-RR (same clone as 86577-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86577-1-RR (Pon3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86577-1-PBS in a different storage buffer formulation.

• 

  Sandwich ELISA sample test of MP02543-1

  Serum of six mice was measured. The PON3 concentration of detected samples was determined to be 19.6 ng/mL with a range of 16.7-21.2 ng/mL.

• 

  Sandwich ELISA sample test of MP02543-1

  The mean PON3 concentration was determined to be 41.6 ng/mL in RAW 264.7 cell extract based on a 1.3 mg/mL extract load and 1,033.8 ng/mL in mouse liver tissue extract based on a 8.4 mg/mL extract load.

• 

  Sandwich ELISA standard curve of MP02543-1

  Sandwich ELISA standard curve of MP02543-1, Mouse PON3 Recombinant Matched Antibody Pair - PBS only. 86577-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg2743. 86577-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

• 

  Cytometric bead array sample test of MP02543-1

  The mean PON3 concentration was determined to be 986.3 ng/mL in Mouse liver tissue extract based on a 8.4 mg/mL extract load.

• 

  Cytometric bead array standard curve of MP02543-1

  Cytometric bead array standard curve of MP02543-1, MOUSE PON3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86577-2-PBS. Detection antibody: 86577-1-PBS. Standard: Eg2743. Range: 0.469-60 ng/mL.

---

Informations sur le produit

86577-1-PBS cible Pon3 dans les applications de WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Pon3 Protéine recombinante Eg2743
• Nom complet: paraoxonase 3
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 40 kDa
• Numéro d’acquisition GenBank: NM_173006.1
• Symbole du gène: Pon3
• Identification du gène (NCBI): 269823
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PON3 (paraoxonase 3) is a member of the paraoxonase gene family and shares high homology with PON1 and PON2. The PON3 protein is primarily synthesized by the liver and can bind to high-density lipoprotein (HDL), where it exerts antioxidant functions. PON3 can prevent the oxidation of low-density lipoprotein (LDL) and disrupt bacterial quorum-sensing molecules. Additionally, PON3 is expressed in mouse models of Alzheimer's disease (AD), with positive staining for PON3 detected in astrocytes surrounding Aβ plaques. These findings suggest that PON3 plays an important role in antioxidant stress and lipid peroxidation and may have potential protective effects on pathological processes such as neurodegenerative diseases and atherosclerosis.

{{ptg:RelatedPrimaryAntibodies}}